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anti human cntf antibody titers  (R&D Systems)


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    R&D Systems anti human cntf antibody titers
    Ciliary neurotrophic factor <t>(CNTF)</t> pharmacokinetics from explanted revakinagene taroretcel-lwey devices. Secretion of CNTF (ng/day) by revakinagene taroretcel-lwey devices explanted over 14.5 years of implant durations, grouped to the nearest half-year for durations <3 years. The solid line indicates the weighted mean CNTF output level, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; ng, nanogram.
    Anti Human Cntf Antibody Titers, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cntf/Human+CNTF+Antibody/pmc12327535-45-8-22
    Average 85 stars, based on 8 article reviews
    anti human cntf antibody titers - by Bioz Stars, 2026-09
    85/100 stars

    Images

    1) Product Images from "Long-Term Durability of Ciliary Neurotrophic Factor–Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders"

    Article Title: Long-Term Durability of Ciliary Neurotrophic Factor–Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.66.11.3

    Ciliary neurotrophic factor (CNTF) pharmacokinetics from explanted revakinagene taroretcel-lwey devices. Secretion of CNTF (ng/day) by revakinagene taroretcel-lwey devices explanted over 14.5 years of implant durations, grouped to the nearest half-year for durations <3 years. The solid line indicates the weighted mean CNTF output level, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; ng, nanogram.
    Figure Legend Snippet: Ciliary neurotrophic factor (CNTF) pharmacokinetics from explanted revakinagene taroretcel-lwey devices. Secretion of CNTF (ng/day) by revakinagene taroretcel-lwey devices explanted over 14.5 years of implant durations, grouped to the nearest half-year for durations <3 years. The solid line indicates the weighted mean CNTF output level, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; ng, nanogram.

    Techniques Used: Drug discovery

    Units of activity of CNTF produced by revakinagene taroretcel-lwey explants over 14.5 years of implant duration. Seven samples were analyzed, 2 of which were analyzed twice, and the results were averaged. The solid line indicates the slope of units of activity, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; EC 50 , half-maximal effective concentration.
    Figure Legend Snippet: Units of activity of CNTF produced by revakinagene taroretcel-lwey explants over 14.5 years of implant duration. Seven samples were analyzed, 2 of which were analyzed twice, and the results were averaged. The solid line indicates the slope of units of activity, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; EC 50 , half-maximal effective concentration.

    Techniques Used: Activity Assay, Produced, Concentration Assay

    Electropherograms of CNTF from explanted revakinagene taroretcel-lwey. ( A ) Electropherograms and ( B ) corresponding bands from CNTF-conditioned media collected from devices explanted from 6 participants over 14.5 years. The explant-derived CNTF is a mixture of 29 kDa human CNTF and the 33 kDa glycosylated CNTF unique to NTC-201-6A cells. Electropherograms of ( C ) controls and ( D ) corresponding bands are shown. The controls used in this analysis include purified CNTF produced in bacteria, purified revakinagene taroretcel-lwey–secreted CNTF, and culture media. Purified CNTF protein produced in bacteria consistently has a high MW shoulder. CNTF, ciliary neurotrophic factor; kDa, kilodalton; MW, molecular weight.
    Figure Legend Snippet: Electropherograms of CNTF from explanted revakinagene taroretcel-lwey. ( A ) Electropherograms and ( B ) corresponding bands from CNTF-conditioned media collected from devices explanted from 6 participants over 14.5 years. The explant-derived CNTF is a mixture of 29 kDa human CNTF and the 33 kDa glycosylated CNTF unique to NTC-201-6A cells. Electropherograms of ( C ) controls and ( D ) corresponding bands are shown. The controls used in this analysis include purified CNTF produced in bacteria, purified revakinagene taroretcel-lwey–secreted CNTF, and culture media. Purified CNTF protein produced in bacteria consistently has a high MW shoulder. CNTF, ciliary neurotrophic factor; kDa, kilodalton; MW, molecular weight.

    Techniques Used: Derivative Assay, Purification, Produced, Bacteria, Molecular Weight

    Related Articles

    Immunofluorescence:

    Article Title: Neurotrophic interactions between neurons and astrocytes following AAV1-Rheb(S16H) transduction in the hippocampus in vivo.
    Article Snippet: For Nissl staining, brain sections were mounted on gelatin‐coated slides, stained with 0.5% cresyl violet (Cat No. C5042; Sigma) and analysed using a bright‐field microscope (RRID:SCR_016980; Axio Imager; Carl Zeiss). .. For immunofluorescence labelling, brain sections were rinsed and incubated for 48 hr with one of the following pairs: rabbit anti‐FLAG (1:3,000; Cat No. F7425; RRID:AB_439687; Sigma) and mouse anti‐ NeuN (1:500; Millipore), rabbit anti‐FLAG (1:3,000; Sigma) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐FLAG (1:2,000; Cat No. F3165; RRID:AB_259529; Sigma) and rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries), mouse anti‐NeuN (1:500; Millipore) and rabbit anti‐BDNF (1:200; Santa Cruz Biotechnology), rabbit anti‐GFAP (1:2,000; Millipore) and goat anti‐TrkB (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Cat No. MCA275GA; RRID:AB_566455; Serotec, Oxford, UK) and goat anti‐TrkB (1:100; R&D Systems), rabbit anti‐p‐ 4E‐BP1 (1:1,000; Cell Signaling Technology) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTF (1:100; R&D Systems), mouse anti‐NeuN (1:500; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and rabbit anti‐p‐ 4E‐BP1 (1:1,000; Cell Signaling Technology), rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries) and goat anti‐CNTF (1:100; R&D Systems), or mouse anti‐microtubule‐associated protein 2 (MAP 2; 1:500; Cat No. MAB3418; RRID:AB_94856; Millipore) and goat anti‐TrkB (1:100; R&D Systems). .. The sections were then rinsed, incubated with Texas Red‐conjugated second antibodies: goat anti‐rabbit IgG (1:400; Cat No. TI‐1000; RRID:AB_2336199; Vector Laboratories), rabbit anti‐goat IgG (1:400; Cat No. TI‐5000; RRID: AB_2336129; Vector Laboratories), horse anti‐mouse IgG (1:400; Cat No. TI‐2000; RRID:AB_2336178; Vector Laboratories), fluorescein isothiocyanate‐conjugated donkey anti‐rabbit IgG (1:200; Cat No. 711‐095‐152; RRID:AB_2315776; Jackson Lab, West Grove, PA, USA), or horse anti‐mouse IgG (1:200; Cat No. FI‐2000; RRID: AB_2336176; Vector Laboratories) for 1 hr, washed and mounted with Vectashield mounting medium (Cat No. H‐1000; RRID: AB_2336789; Vector Laboratories).

    Article Title: Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo
    Article Snippet: For Nissl staining, brain sections were mounted on gelatin‐coated slides, stained with 0.5% cresyl violet (Cat No. C5042; Sigma) and analysed using a bright‐field microscope (RRID:SCR_016980; Axio Imager; Carl Zeiss). .. For immunofluorescence labelling, brain sections were rinsed and incubated for 48 hr with one of the following pairs: rabbit anti‐FLAG (1:3,000; Cat No. F7425; RRID:AB_439687; Sigma) and mouse anti‐NeuN (1:500; Millipore), rabbit anti‐FLAG (1:3,000; Sigma) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐FLAG (1:2,000; Cat No. F3165; RRID:AB_259529; Sigma) and rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries), mouse anti‐NeuN (1:500; Millipore) and rabbit anti‐BDNF (1:200; Santa Cruz Biotechnology), rabbit anti‐GFAP (1:2,000; Millipore) and goat anti‐TrkB (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Cat No. MCA275GA; RRID:AB_566455; Serotec, Oxford, UK) and goat anti‐TrkB (1:100; R&D Systems), rabbit anti‐p‐4E‐BP1 (1:1,000; Cell Signaling Technology) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTF (1:100; R&D Systems), mouse anti‐NeuN (1:500; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and rabbit anti‐p‐4E‐BP1 (1:1,000; Cell Signaling Technology), rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries) and goat anti‐CNTF (1:100; R&D Systems), or mouse anti‐microtubule‐associated protein 2 (MAP 2; 1:500; Cat No. MAB3418; RRID:AB_94856; Millipore) and goat anti‐TrkB (1:100; R&D Systems). .. The sections were then rinsed, incubated with Texas Red‐conjugated second antibodies: goat anti‐rabbit IgG (1:400; Cat No. TI‐1000; RRID:AB_2336199; Vector Laboratories), rabbit anti‐goat IgG (1:400; Cat No. TI‐5000; RRID:AB_2336129; Vector Laboratories), horse anti‐mouse IgG (1:400; Cat No. TI‐2000; RRID:AB_2336178; Vector Laboratories), fluorescein isothiocyanate‐conjugated donkey anti‐rabbit IgG (1:200; Cat No. 711‐095‐152; RRID:AB_2315776; Jackson Lab, West Grove, PA, USA), or horse anti‐mouse IgG (1:200; Cat No. FI‐2000; RRID:AB_2336176; Vector Laboratories) for 1 hr, washed and mounted with Vectashield mounting medium (Cat No. H‐1000; RRID:AB_2336789; Vector Laboratories).

    Incubation:

    Article Title: Neurotrophic interactions between neurons and astrocytes following AAV1-Rheb(S16H) transduction in the hippocampus in vivo.
    Article Snippet: For Nissl staining, brain sections were mounted on gelatin‐coated slides, stained with 0.5% cresyl violet (Cat No. C5042; Sigma) and analysed using a bright‐field microscope (RRID:SCR_016980; Axio Imager; Carl Zeiss). .. For immunofluorescence labelling, brain sections were rinsed and incubated for 48 hr with one of the following pairs: rabbit anti‐FLAG (1:3,000; Cat No. F7425; RRID:AB_439687; Sigma) and mouse anti‐ NeuN (1:500; Millipore), rabbit anti‐FLAG (1:3,000; Sigma) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐FLAG (1:2,000; Cat No. F3165; RRID:AB_259529; Sigma) and rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries), mouse anti‐NeuN (1:500; Millipore) and rabbit anti‐BDNF (1:200; Santa Cruz Biotechnology), rabbit anti‐GFAP (1:2,000; Millipore) and goat anti‐TrkB (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Cat No. MCA275GA; RRID:AB_566455; Serotec, Oxford, UK) and goat anti‐TrkB (1:100; R&D Systems), rabbit anti‐p‐ 4E‐BP1 (1:1,000; Cell Signaling Technology) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTF (1:100; R&D Systems), mouse anti‐NeuN (1:500; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and rabbit anti‐p‐ 4E‐BP1 (1:1,000; Cell Signaling Technology), rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries) and goat anti‐CNTF (1:100; R&D Systems), or mouse anti‐microtubule‐associated protein 2 (MAP 2; 1:500; Cat No. MAB3418; RRID:AB_94856; Millipore) and goat anti‐TrkB (1:100; R&D Systems). .. The sections were then rinsed, incubated with Texas Red‐conjugated second antibodies: goat anti‐rabbit IgG (1:400; Cat No. TI‐1000; RRID:AB_2336199; Vector Laboratories), rabbit anti‐goat IgG (1:400; Cat No. TI‐5000; RRID: AB_2336129; Vector Laboratories), horse anti‐mouse IgG (1:400; Cat No. TI‐2000; RRID:AB_2336178; Vector Laboratories), fluorescein isothiocyanate‐conjugated donkey anti‐rabbit IgG (1:200; Cat No. 711‐095‐152; RRID:AB_2315776; Jackson Lab, West Grove, PA, USA), or horse anti‐mouse IgG (1:200; Cat No. FI‐2000; RRID: AB_2336176; Vector Laboratories) for 1 hr, washed and mounted with Vectashield mounting medium (Cat No. H‐1000; RRID: AB_2336789; Vector Laboratories).

    Article Title: Dl-3-n-Butylphthalide Alleviates Hippocampal Neuron Damage in Chronic Cerebral Hypoperfusion via Regulation of the CNTF/CNTFRα/JAK2/STAT3 Signaling Pathways
    Article Snippet: .. Then, the membranes were blocked for 2 h at room temperature in a blocking buffer containing 5% fat-free milk and incubated with the following primary antibodies: anti-CNTF (1:300; R&D systems, Minneapolis, MN, USA), anti-CNTFRα (1:300; R&D systems, Minneapolis, MN, USA), anti-t-JAK2 (1:300; Abcam, Cambridge, MA, USA), anti-p-JAK2 (Tyr1007/1008; 1:300; Abcam, Cambridge, MA, USA), anti-t-STAT3 (1:300; Abcam, Cambridge, MA, USA), anti-p-STAT3 (Tyr705; 1:300; Abcam, Cambridge, MA, USA), and anti-β-actin (1:500; Abcam, Cambridge, MA, USA), which was used as a loading control ( ). .. The next day, the membranes were washed three times with Tris-buffered saline that contained Tween 20 and incubated with the secondary antibody (1:10,000; Abcam, Cambridge, MA, USA) for 1 h. The bands on the membranes were detected using the enhanced chemiluminescence (ECL; Thermo Fisher Scientific, Waltham, MA, USA) method and quantified using ImageJ analysis software (National Institutes of Health, Bethesda, MD, USA; Niu et al., ).

    Article Title: Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo
    Article Snippet: For Nissl staining, brain sections were mounted on gelatin‐coated slides, stained with 0.5% cresyl violet (Cat No. C5042; Sigma) and analysed using a bright‐field microscope (RRID:SCR_016980; Axio Imager; Carl Zeiss). .. For immunofluorescence labelling, brain sections were rinsed and incubated for 48 hr with one of the following pairs: rabbit anti‐FLAG (1:3,000; Cat No. F7425; RRID:AB_439687; Sigma) and mouse anti‐NeuN (1:500; Millipore), rabbit anti‐FLAG (1:3,000; Sigma) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐FLAG (1:2,000; Cat No. F3165; RRID:AB_259529; Sigma) and rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries), mouse anti‐NeuN (1:500; Millipore) and rabbit anti‐BDNF (1:200; Santa Cruz Biotechnology), rabbit anti‐GFAP (1:2,000; Millipore) and goat anti‐TrkB (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Cat No. MCA275GA; RRID:AB_566455; Serotec, Oxford, UK) and goat anti‐TrkB (1:100; R&D Systems), rabbit anti‐p‐4E‐BP1 (1:1,000; Cell Signaling Technology) and mouse anti‐GFAP (1:2,000; Millipore), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTF (1:100; R&D Systems), mouse anti‐NeuN (1:500; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐GFAP (1:2,000; Millipore) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and goat anti‐CNTFRα (1:100; R&D Systems), mouse anti‐OX‐42 (1:500; Serotec) and rabbit anti‐p‐4E‐BP1 (1:1,000; Cell Signaling Technology), rabbit anti‐Iba1 (1:2,000; Wako Pure Chemical Industries) and goat anti‐CNTF (1:100; R&D Systems), or mouse anti‐microtubule‐associated protein 2 (MAP 2; 1:500; Cat No. MAB3418; RRID:AB_94856; Millipore) and goat anti‐TrkB (1:100; R&D Systems). .. The sections were then rinsed, incubated with Texas Red‐conjugated second antibodies: goat anti‐rabbit IgG (1:400; Cat No. TI‐1000; RRID:AB_2336199; Vector Laboratories), rabbit anti‐goat IgG (1:400; Cat No. TI‐5000; RRID:AB_2336129; Vector Laboratories), horse anti‐mouse IgG (1:400; Cat No. TI‐2000; RRID:AB_2336178; Vector Laboratories), fluorescein isothiocyanate‐conjugated donkey anti‐rabbit IgG (1:200; Cat No. 711‐095‐152; RRID:AB_2315776; Jackson Lab, West Grove, PA, USA), or horse anti‐mouse IgG (1:200; Cat No. FI‐2000; RRID:AB_2336176; Vector Laboratories) for 1 hr, washed and mounted with Vectashield mounting medium (Cat No. H‐1000; RRID:AB_2336789; Vector Laboratories).

    Blocking Assay:

    Article Title: Dl-3-n-Butylphthalide Alleviates Hippocampal Neuron Damage in Chronic Cerebral Hypoperfusion via Regulation of the CNTF/CNTFRα/JAK2/STAT3 Signaling Pathways
    Article Snippet: .. Then, the membranes were blocked for 2 h at room temperature in a blocking buffer containing 5% fat-free milk and incubated with the following primary antibodies: anti-CNTF (1:300; R&D systems, Minneapolis, MN, USA), anti-CNTFRα (1:300; R&D systems, Minneapolis, MN, USA), anti-t-JAK2 (1:300; Abcam, Cambridge, MA, USA), anti-p-JAK2 (Tyr1007/1008; 1:300; Abcam, Cambridge, MA, USA), anti-t-STAT3 (1:300; Abcam, Cambridge, MA, USA), anti-p-STAT3 (Tyr705; 1:300; Abcam, Cambridge, MA, USA), and anti-β-actin (1:500; Abcam, Cambridge, MA, USA), which was used as a loading control ( ). .. The next day, the membranes were washed three times with Tris-buffered saline that contained Tween 20 and incubated with the secondary antibody (1:10,000; Abcam, Cambridge, MA, USA) for 1 h. The bands on the membranes were detected using the enhanced chemiluminescence (ECL; Thermo Fisher Scientific, Waltham, MA, USA) method and quantified using ImageJ analysis software (National Institutes of Health, Bethesda, MD, USA; Niu et al., ).

    Control:

    Article Title: Dl-3-n-Butylphthalide Alleviates Hippocampal Neuron Damage in Chronic Cerebral Hypoperfusion via Regulation of the CNTF/CNTFRα/JAK2/STAT3 Signaling Pathways
    Article Snippet: .. Then, the membranes were blocked for 2 h at room temperature in a blocking buffer containing 5% fat-free milk and incubated with the following primary antibodies: anti-CNTF (1:300; R&D systems, Minneapolis, MN, USA), anti-CNTFRα (1:300; R&D systems, Minneapolis, MN, USA), anti-t-JAK2 (1:300; Abcam, Cambridge, MA, USA), anti-p-JAK2 (Tyr1007/1008; 1:300; Abcam, Cambridge, MA, USA), anti-t-STAT3 (1:300; Abcam, Cambridge, MA, USA), anti-p-STAT3 (Tyr705; 1:300; Abcam, Cambridge, MA, USA), and anti-β-actin (1:500; Abcam, Cambridge, MA, USA), which was used as a loading control ( ). .. The next day, the membranes were washed three times with Tris-buffered saline that contained Tween 20 and incubated with the secondary antibody (1:10,000; Abcam, Cambridge, MA, USA) for 1 h. The bands on the membranes were detected using the enhanced chemiluminescence (ECL; Thermo Fisher Scientific, Waltham, MA, USA) method and quantified using ImageJ analysis software (National Institutes of Health, Bethesda, MD, USA; Niu et al., ).



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    Santa Cruz Biotechnology cntf
    Figure 2. (A) Survival and differentiation of intravitreally transplanted NSCs. Analyses of experimen- tal eyes 51 days after cell transplantation revealed the presence of reporter protein-positive donor cells on the posterior surface of the lenses (A,B). While all control-NSCs (Aa–Ac), <t>CNTF-NSCs</t> (Ad–Af) <t>and</t> <t>GDNF-NSCs</t> (Ag–Ai) differentiated into GFAP-positive astrocytes, a fraction of CNTF/GDNF- NSCs (Aj–Al) additionally differentiated into β-TUB III–positive neurons. (B) Transgene expression in intravitreally transplanted NSCs. Control-NSCs expressed the reporter protein Venus (Ba) but not GDNF (Bb) or CNTF (Bc). CNTF/GDNF-NSCs co-expressed the reporter proteins eGFP or Venus (Bd) and GDNF (Be) or CNTF (Bf), respectively. CNTF, ciliary neurotrophic factor; eGFP, enhanced green fluorescent protein; GDNF, glial cell line-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; NSCs, neural stem cells; β-TUB III, class III β-tubulin. Scale bars: 100 µm.
    Cntf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cntf  (Proteintech)
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    Proteintech cntf
    Figure 2. (A) Survival and differentiation of intravitreally transplanted NSCs. Analyses of experimen- tal eyes 51 days after cell transplantation revealed the presence of reporter protein-positive donor cells on the posterior surface of the lenses (A,B). While all control-NSCs (Aa–Ac), <t>CNTF-NSCs</t> (Ad–Af) <t>and</t> <t>GDNF-NSCs</t> (Ag–Ai) differentiated into GFAP-positive astrocytes, a fraction of CNTF/GDNF- NSCs (Aj–Al) additionally differentiated into β-TUB III–positive neurons. (B) Transgene expression in intravitreally transplanted NSCs. Control-NSCs expressed the reporter protein Venus (Ba) but not GDNF (Bb) or CNTF (Bc). CNTF/GDNF-NSCs co-expressed the reporter proteins eGFP or Venus (Bd) and GDNF (Be) or CNTF (Bf), respectively. CNTF, ciliary neurotrophic factor; eGFP, enhanced green fluorescent protein; GDNF, glial cell line-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; NSCs, neural stem cells; β-TUB III, class III β-tubulin. Scale bars: 100 µm.
    Cntf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cntf/CNTF+Antibody/pm40082289-56-11-16
    Average 93 stars, based on 1 article reviews
    cntf - by Bioz Stars, 2026-09
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    Image Search Results


    Ciliary neurotrophic factor (CNTF) pharmacokinetics from explanted revakinagene taroretcel-lwey devices. Secretion of CNTF (ng/day) by revakinagene taroretcel-lwey devices explanted over 14.5 years of implant durations, grouped to the nearest half-year for durations <3 years. The solid line indicates the weighted mean CNTF output level, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; ng, nanogram.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Long-Term Durability of Ciliary Neurotrophic Factor–Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders

    doi: 10.1167/iovs.66.11.3

    Figure Lengend Snippet: Ciliary neurotrophic factor (CNTF) pharmacokinetics from explanted revakinagene taroretcel-lwey devices. Secretion of CNTF (ng/day) by revakinagene taroretcel-lwey devices explanted over 14.5 years of implant durations, grouped to the nearest half-year for durations <3 years. The solid line indicates the weighted mean CNTF output level, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; ng, nanogram.

    Article Snippet: An ELISA was also used to quantify specific anti-human CNTF antibody titers after incubating participant serum on a plate coated with rhCNTF (R&D Systems).

    Techniques: Drug discovery

    Units of activity of CNTF produced by revakinagene taroretcel-lwey explants over 14.5 years of implant duration. Seven samples were analyzed, 2 of which were analyzed twice, and the results were averaged. The solid line indicates the slope of units of activity, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; EC 50 , half-maximal effective concentration.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Long-Term Durability of Ciliary Neurotrophic Factor–Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders

    doi: 10.1167/iovs.66.11.3

    Figure Lengend Snippet: Units of activity of CNTF produced by revakinagene taroretcel-lwey explants over 14.5 years of implant duration. Seven samples were analyzed, 2 of which were analyzed twice, and the results were averaged. The solid line indicates the slope of units of activity, and the dotted lines indicate the range of the 95% confidence interval of the slope. CNTF, ciliary neurotrophic factor; EC 50 , half-maximal effective concentration.

    Article Snippet: An ELISA was also used to quantify specific anti-human CNTF antibody titers after incubating participant serum on a plate coated with rhCNTF (R&D Systems).

    Techniques: Activity Assay, Produced, Concentration Assay

    Electropherograms of CNTF from explanted revakinagene taroretcel-lwey. ( A ) Electropherograms and ( B ) corresponding bands from CNTF-conditioned media collected from devices explanted from 6 participants over 14.5 years. The explant-derived CNTF is a mixture of 29 kDa human CNTF and the 33 kDa glycosylated CNTF unique to NTC-201-6A cells. Electropherograms of ( C ) controls and ( D ) corresponding bands are shown. The controls used in this analysis include purified CNTF produced in bacteria, purified revakinagene taroretcel-lwey–secreted CNTF, and culture media. Purified CNTF protein produced in bacteria consistently has a high MW shoulder. CNTF, ciliary neurotrophic factor; kDa, kilodalton; MW, molecular weight.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Long-Term Durability of Ciliary Neurotrophic Factor–Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders

    doi: 10.1167/iovs.66.11.3

    Figure Lengend Snippet: Electropherograms of CNTF from explanted revakinagene taroretcel-lwey. ( A ) Electropherograms and ( B ) corresponding bands from CNTF-conditioned media collected from devices explanted from 6 participants over 14.5 years. The explant-derived CNTF is a mixture of 29 kDa human CNTF and the 33 kDa glycosylated CNTF unique to NTC-201-6A cells. Electropherograms of ( C ) controls and ( D ) corresponding bands are shown. The controls used in this analysis include purified CNTF produced in bacteria, purified revakinagene taroretcel-lwey–secreted CNTF, and culture media. Purified CNTF protein produced in bacteria consistently has a high MW shoulder. CNTF, ciliary neurotrophic factor; kDa, kilodalton; MW, molecular weight.

    Article Snippet: An ELISA was also used to quantify specific anti-human CNTF antibody titers after incubating participant serum on a plate coated with rhCNTF (R&D Systems).

    Techniques: Derivative Assay, Purification, Produced, Bacteria, Molecular Weight

    Figure 2. (A) Survival and differentiation of intravitreally transplanted NSCs. Analyses of experimen- tal eyes 51 days after cell transplantation revealed the presence of reporter protein-positive donor cells on the posterior surface of the lenses (A,B). While all control-NSCs (Aa–Ac), CNTF-NSCs (Ad–Af) and GDNF-NSCs (Ag–Ai) differentiated into GFAP-positive astrocytes, a fraction of CNTF/GDNF- NSCs (Aj–Al) additionally differentiated into β-TUB III–positive neurons. (B) Transgene expression in intravitreally transplanted NSCs. Control-NSCs expressed the reporter protein Venus (Ba) but not GDNF (Bb) or CNTF (Bc). CNTF/GDNF-NSCs co-expressed the reporter proteins eGFP or Venus (Bd) and GDNF (Be) or CNTF (Bf), respectively. CNTF, ciliary neurotrophic factor; eGFP, enhanced green fluorescent protein; GDNF, glial cell line-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; NSCs, neural stem cells; β-TUB III, class III β-tubulin. Scale bars: 100 µm.

    Journal: Cells

    Article Title: Survival and Axonal Regeneration of Retinal Ganglion Cells in a Mouse Optic Nerve Crush Model After a Cell-Based Intravitreal Co-Administration of Ciliary Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor at Different Post-Lesion Time Points.

    doi: 10.3390/cells14090643

    Figure Lengend Snippet: Figure 2. (A) Survival and differentiation of intravitreally transplanted NSCs. Analyses of experimen- tal eyes 51 days after cell transplantation revealed the presence of reporter protein-positive donor cells on the posterior surface of the lenses (A,B). While all control-NSCs (Aa–Ac), CNTF-NSCs (Ad–Af) and GDNF-NSCs (Ag–Ai) differentiated into GFAP-positive astrocytes, a fraction of CNTF/GDNF- NSCs (Aj–Al) additionally differentiated into β-TUB III–positive neurons. (B) Transgene expression in intravitreally transplanted NSCs. Control-NSCs expressed the reporter protein Venus (Ba) but not GDNF (Bb) or CNTF (Bc). CNTF/GDNF-NSCs co-expressed the reporter proteins eGFP or Venus (Bd) and GDNF (Be) or CNTF (Bf), respectively. CNTF, ciliary neurotrophic factor; eGFP, enhanced green fluorescent protein; GDNF, glial cell line-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; NSCs, neural stem cells; β-TUB III, class III β-tubulin. Scale bars: 100 µm.

    Article Snippet: After further fixation for 15 min and blocking for 1 h, lenses with attached donor cells were simultaneously immunostained with antibodies against CNTF (1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and GDNF (1:50; R&D Systems, Inc., Minneapolis, MN, USA) to analyze the expression of the neuroprotective factors, or with antibodies against glial fibrillary acidic protein (GFAP,1:500; Agilent Technologies, Inc., Santa Clara, CA, USA) and class III β-tubulin (β-TUB III, 1:1000; Sigma-Aldrich, St. Louis, MO, USA) to monitor the differentiation of the donor cells.

    Techniques: Transplantation Assay, Control, Expressing, Derivative Assay

    Figure 3. (A) Representative micrographs of retinal flatmounts from animals that had received NSC transplantations 3 days after ONC. The density of BRN-3A-positive RGCs in CNTF-treated (Ab,Af,Aj) and GDNF-treated (Ac,Ag,Ak) retinas was significantly higher than in control retinas (Aa,Ae,Ai) 14, 28 and 56 dpl. Note the significantly higher number of surviving RGCs at all post-lesion time points in retinas co-treated with CNTF and GDNF (Ad,Ah,Al). (B) Quantitative analysis of RGC survival. Each bar represents the mean value (±SEM) of six animals. ns: not significant; ***: p < 0.001 according to a two-way ANOVA followed by a Bonferroni post-hoc test. BRN-3A, brain-specific homeobox/POU domain protein 3A; CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush; RGCs, retinal ganglion cells. Scale bar: 100 µm.

    Journal: Cells

    Article Title: Survival and Axonal Regeneration of Retinal Ganglion Cells in a Mouse Optic Nerve Crush Model After a Cell-Based Intravitreal Co-Administration of Ciliary Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor at Different Post-Lesion Time Points.

    doi: 10.3390/cells14090643

    Figure Lengend Snippet: Figure 3. (A) Representative micrographs of retinal flatmounts from animals that had received NSC transplantations 3 days after ONC. The density of BRN-3A-positive RGCs in CNTF-treated (Ab,Af,Aj) and GDNF-treated (Ac,Ag,Ak) retinas was significantly higher than in control retinas (Aa,Ae,Ai) 14, 28 and 56 dpl. Note the significantly higher number of surviving RGCs at all post-lesion time points in retinas co-treated with CNTF and GDNF (Ad,Ah,Al). (B) Quantitative analysis of RGC survival. Each bar represents the mean value (±SEM) of six animals. ns: not significant; ***: p < 0.001 according to a two-way ANOVA followed by a Bonferroni post-hoc test. BRN-3A, brain-specific homeobox/POU domain protein 3A; CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush; RGCs, retinal ganglion cells. Scale bar: 100 µm.

    Article Snippet: After further fixation for 15 min and blocking for 1 h, lenses with attached donor cells were simultaneously immunostained with antibodies against CNTF (1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and GDNF (1:50; R&D Systems, Inc., Minneapolis, MN, USA) to analyze the expression of the neuroprotective factors, or with antibodies against glial fibrillary acidic protein (GFAP,1:500; Agilent Technologies, Inc., Santa Clara, CA, USA) and class III β-tubulin (β-TUB III, 1:1000; Sigma-Aldrich, St. Louis, MO, USA) to monitor the differentiation of the donor cells.

    Techniques: Control, Derivative Assay

    Figure 4. (A) Representative micrographs of retinal flatmounts from animals that had received NSC transplantations 5 days after ONC. The density of BRN-3A-positive RGCs in CNTF-treated (Ab,Af,Aj) and GDNF-treated (Ac,Ag,Ak) retinas was significantly higher than in control retinas (Aa,Ae,Ai) 14, 28 and 56 dpl. Note the significantly higher number of surviving RGCs at all post-lesion time points in retinas co-treated with CNTF and GDNF (Ad,Ah,Al). (B) Quantitative analysis of RGC survival. Each bar represents the mean value (±SEM) of six animals. ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001 according to a two-way ANOVA followed by a Bonferroni post-hoc test. BRN-3A, brain-specific homeobox/POU domain protein 3A; CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line–derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush; RGCs, retinal ganglion cells. Scale bar: 100 µm.

    Journal: Cells

    Article Title: Survival and Axonal Regeneration of Retinal Ganglion Cells in a Mouse Optic Nerve Crush Model After a Cell-Based Intravitreal Co-Administration of Ciliary Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor at Different Post-Lesion Time Points.

    doi: 10.3390/cells14090643

    Figure Lengend Snippet: Figure 4. (A) Representative micrographs of retinal flatmounts from animals that had received NSC transplantations 5 days after ONC. The density of BRN-3A-positive RGCs in CNTF-treated (Ab,Af,Aj) and GDNF-treated (Ac,Ag,Ak) retinas was significantly higher than in control retinas (Aa,Ae,Ai) 14, 28 and 56 dpl. Note the significantly higher number of surviving RGCs at all post-lesion time points in retinas co-treated with CNTF and GDNF (Ad,Ah,Al). (B) Quantitative analysis of RGC survival. Each bar represents the mean value (±SEM) of six animals. ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001 according to a two-way ANOVA followed by a Bonferroni post-hoc test. BRN-3A, brain-specific homeobox/POU domain protein 3A; CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line–derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush; RGCs, retinal ganglion cells. Scale bar: 100 µm.

    Article Snippet: After further fixation for 15 min and blocking for 1 h, lenses with attached donor cells were simultaneously immunostained with antibodies against CNTF (1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and GDNF (1:50; R&D Systems, Inc., Minneapolis, MN, USA) to analyze the expression of the neuroprotective factors, or with antibodies against glial fibrillary acidic protein (GFAP,1:500; Agilent Technologies, Inc., Santa Clara, CA, USA) and class III β-tubulin (β-TUB III, 1:1000; Sigma-Aldrich, St. Louis, MO, USA) to monitor the differentiation of the donor cells.

    Techniques: Control, Derivative Assay

    Figure 5. Axonal regeneration in animals that had received NSC transplantations 5 days after ONC. Analyses of animals with grafted control-NSCs (a) or GDNF-NSCs (b) 28 dpl revealed the presence of only a few regrown axons extending only a short distance beyond the lesion site. Animals with grafted CNTF-NSCs (c) or CNTF/GDNF-NSCs (d), in comparison, contained significantly more and longer regrown axons in the distal nerve stumps. Axons displayed an irregular course, and some made U-turns (arrows in (c,d)). CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush. Scale bar: 200 µm.

    Journal: Cells

    Article Title: Survival and Axonal Regeneration of Retinal Ganglion Cells in a Mouse Optic Nerve Crush Model After a Cell-Based Intravitreal Co-Administration of Ciliary Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor at Different Post-Lesion Time Points.

    doi: 10.3390/cells14090643

    Figure Lengend Snippet: Figure 5. Axonal regeneration in animals that had received NSC transplantations 5 days after ONC. Analyses of animals with grafted control-NSCs (a) or GDNF-NSCs (b) 28 dpl revealed the presence of only a few regrown axons extending only a short distance beyond the lesion site. Animals with grafted CNTF-NSCs (c) or CNTF/GDNF-NSCs (d), in comparison, contained significantly more and longer regrown axons in the distal nerve stumps. Axons displayed an irregular course, and some made U-turns (arrows in (c,d)). CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush. Scale bar: 200 µm.

    Article Snippet: After further fixation for 15 min and blocking for 1 h, lenses with attached donor cells were simultaneously immunostained with antibodies against CNTF (1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and GDNF (1:50; R&D Systems, Inc., Minneapolis, MN, USA) to analyze the expression of the neuroprotective factors, or with antibodies against glial fibrillary acidic protein (GFAP,1:500; Agilent Technologies, Inc., Santa Clara, CA, USA) and class III β-tubulin (β-TUB III, 1:1000; Sigma-Aldrich, St. Louis, MO, USA) to monitor the differentiation of the donor cells.

    Techniques: Control, Comparison, Derivative Assay

    Figure 6. Quantitative analysis of axonal regeneration in animals that had received NSC transplan- tations 5 days after ONC. The length of the longest regrown axon 28 dpl beyond the lesion site in animals treated with control-NSCs, GDNF-NSCs, CNTF-NSCs or CNTF/GDNF-NSCs (a). Each horizontal line represents the mean values of six animals. ns: not significant; ***: p < 0.001 according to a one-way ANOVA followed by a Bonferroni post-hoc test. The number of regrown axons in CNTF-treated and CNTF/GDNF-treated mice at different positions distal to the lesion site 28 dpl (b). Each bar represents the mean value (±SEM) of six animals. CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush.

    Journal: Cells

    Article Title: Survival and Axonal Regeneration of Retinal Ganglion Cells in a Mouse Optic Nerve Crush Model After a Cell-Based Intravitreal Co-Administration of Ciliary Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor at Different Post-Lesion Time Points.

    doi: 10.3390/cells14090643

    Figure Lengend Snippet: Figure 6. Quantitative analysis of axonal regeneration in animals that had received NSC transplan- tations 5 days after ONC. The length of the longest regrown axon 28 dpl beyond the lesion site in animals treated with control-NSCs, GDNF-NSCs, CNTF-NSCs or CNTF/GDNF-NSCs (a). Each horizontal line represents the mean values of six animals. ns: not significant; ***: p < 0.001 according to a one-way ANOVA followed by a Bonferroni post-hoc test. The number of regrown axons in CNTF-treated and CNTF/GDNF-treated mice at different positions distal to the lesion site 28 dpl (b). Each bar represents the mean value (±SEM) of six animals. CNTF, ciliary neurotrophic factor; dpl, days post-lesion; GDNF, glial cell line-derived neurotrophic factor; NSC, neural stem cell; ONC, optic nerve crush.

    Article Snippet: After further fixation for 15 min and blocking for 1 h, lenses with attached donor cells were simultaneously immunostained with antibodies against CNTF (1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and GDNF (1:50; R&D Systems, Inc., Minneapolis, MN, USA) to analyze the expression of the neuroprotective factors, or with antibodies against glial fibrillary acidic protein (GFAP,1:500; Agilent Technologies, Inc., Santa Clara, CA, USA) and class III β-tubulin (β-TUB III, 1:1000; Sigma-Aldrich, St. Louis, MO, USA) to monitor the differentiation of the donor cells.

    Techniques: Control, Derivative Assay